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cryopreserved human hepatocyte plating medium  (Lonza)


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    Structured Review

    Lonza cryopreserved human hepatocyte plating medium
    Cryopreserved Human Hepatocyte Plating Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryopreserved+human+hepatocyte+plating+medium/hepatocyte+plating+medium/pmc05607027-599-49-41
    Average 90 stars, based on 1 article reviews
    cryopreserved human hepatocyte plating medium - by Bioz Stars, 2026-09
    90/100 stars

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    Sterility:

    Article Title: Microphysiological Systems to Assess Nonclinical Toxicity
    Article Snippet: We adapted and merged the hepatocyte seeding methodology used to seed hepatocytes into the extracellular matrix lumen of the Nortis device with the methods used to create a tube in type I collagen that is used for culturing PTECs. .. Materials Ice bucket with ice (not sterile) Type I collagen (rat tail): Ibidi Cat No. 50204 or Corning Ref# 354249 PTEC media Human Hepatocyte Plating Media Human Hepatocyte Culture/cell washing media Hepatocyte Matrigel Media Medium 199 (10X): Gibco Cat # 21180-021 TRL/Lonza: Cryopreserved Human Hepatocyte Thawing Medium Cat#MCHT50 TRL/Lonza: Cryopreserved Human Hepatocyte Plating Medium CatMP250 (optional) TRL/Lonza: Cryopreserved Human Hepatocyte Maintenance Medium Cat#MM250 (optional) TRL/Lonza: Cryopreserved Human Hepatocytes, plateable induction qualified Cat#HUCPI 1N NaOH Ethanol, 200 proof Becton, Dickinson 1, 3, 5 mL Luer-Lock Tip Syringes Metal Coupler (blunt): Instech Lab, SC 20/15 Single edge razor blade (sterile) Forceps (sterile) 1.5 mL microcentrifuge tubes 15 mL Falcon conical tubes 50 mL conical tubes Matrigel Corning catalog# 354263 Luer-Lock needle blunts 22 ga x 1⁄2′′ stubs (blue): Instech Lab. Cat # LS22 1, 5, 10, 20 mL Luer-Lock Tip Syringes; Becton, Dickinson Silicon tubing: Cole-Parmer C-flex tubing (ID: 0.020", OD: 0.083") Cat# 06422-00 10 cm Petri dishes Equipment Nortis 3D Microphysiological Device (SCC-001) with 120 μm channel Modified Nortis 3D Microphysiological Device (SCC-001) with 450 μm channel Cole Parmer 74900 Series 10-Syringe Infusion Pump or equivalent Benchtop Centrifuge capable of holding 15 and 50 mL conical tubes Humidified Incubator set at 5% CO2 and 37 °C. ..

    Modification:

    Article Title: Microphysiological Systems to Assess Nonclinical Toxicity
    Article Snippet: We adapted and merged the hepatocyte seeding methodology used to seed hepatocytes into the extracellular matrix lumen of the Nortis device with the methods used to create a tube in type I collagen that is used for culturing PTECs. .. Materials Ice bucket with ice (not sterile) Type I collagen (rat tail): Ibidi Cat No. 50204 or Corning Ref# 354249 PTEC media Human Hepatocyte Plating Media Human Hepatocyte Culture/cell washing media Hepatocyte Matrigel Media Medium 199 (10X): Gibco Cat # 21180-021 TRL/Lonza: Cryopreserved Human Hepatocyte Thawing Medium Cat#MCHT50 TRL/Lonza: Cryopreserved Human Hepatocyte Plating Medium CatMP250 (optional) TRL/Lonza: Cryopreserved Human Hepatocyte Maintenance Medium Cat#MM250 (optional) TRL/Lonza: Cryopreserved Human Hepatocytes, plateable induction qualified Cat#HUCPI 1N NaOH Ethanol, 200 proof Becton, Dickinson 1, 3, 5 mL Luer-Lock Tip Syringes Metal Coupler (blunt): Instech Lab, SC 20/15 Single edge razor blade (sterile) Forceps (sterile) 1.5 mL microcentrifuge tubes 15 mL Falcon conical tubes 50 mL conical tubes Matrigel Corning catalog# 354263 Luer-Lock needle blunts 22 ga x 1⁄2′′ stubs (blue): Instech Lab. Cat # LS22 1, 5, 10, 20 mL Luer-Lock Tip Syringes; Becton, Dickinson Silicon tubing: Cole-Parmer C-flex tubing (ID: 0.020", OD: 0.083") Cat# 06422-00 10 cm Petri dishes Equipment Nortis 3D Microphysiological Device (SCC-001) with 120 μm channel Modified Nortis 3D Microphysiological Device (SCC-001) with 450 μm channel Cole Parmer 74900 Series 10-Syringe Infusion Pump or equivalent Benchtop Centrifuge capable of holding 15 and 50 mL conical tubes Humidified Incubator set at 5% CO2 and 37 °C. ..



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